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PBP1 | PYK10-binding protein 1 (N-terminal)
Product no: AS20 4414
AS20 4414 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
369
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- Product Info
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Immunogen: Conjugated peptide, derived from N-terminus of Arabidopsis thaliana PBP1, UniProt: O04314, TAIR: At3g16420 Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized. Format: Liquid at 2 mg/ml. Quantity: 200 µg Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunofluorescence (IF), Western blot (WB) Recommended dilution: 1:1000 (IF), 1: 2000 (WB) Expected | apparent MW: 32 | 35 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica rapa, Raphanus sativus
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Arabidopsis thaliana crude leaf from 7 day-old seedlings was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. 10 µg of protein was loaded and separated on 12.5 % SDS-PAGE and blotted to PVDF membrane at 15 V overnight. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
Arabidopsis thaliana crude leaf from 7 day-old seedlings wilde-type with GFP-h fusion (1), nai1-1 mutant (2), wild-type (3), nai1-2 mutant (4), F1 progeny of GFPhxnail1-2 (5), F1 progeny of nai1-1xnai1-2 (6) was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Protein was loaded and separated on 12.5 % SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
PBP1 protein signal is abolished in sample 2,4,6 that indicates PBP1 expression is regulated by NAI1 gene.
- Background
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Background: PBP1 (PYK10-binding protein 1) is inhibitor-type lectin that may regulate the correct polymerization of BGLU23/PYK10 upon tissue damage. Activates BGLU21, BGLU22 and BGLU23. PBP1 is localised to cytoplasm.
Alternative names: Jacalin-related lectin 30, Jasmonic acid-induced protein. - Product Citations
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Selected references: Nagano et al. (2005). Activation of an ER-body-localized beta-glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana. Plant Cell Physiol. 2005 Jul;46(7):1140-8. doi: 10.1093/pcp/pci126. (Immunofluorescence, Western blot, Arabidopsis thaliana)
Matsushima et al. (2004). NAI1 gene encodes a basic-helix-loop-helix-type putative transcription factor that regulates the formation of an endoplasmic reticulum-derived structure, the ER body. Plant Cell. 2004 Jun;16(6):1536-49. doi: 10.1105/tpc.021154. (Western blot, Arabidopsis thaliana) - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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