Technical information

Antibody types

Purification

Other

Technical information > Molecular weight and isoelectric point of various animal immunoglobulins

Immunoglobulin	Approximate MW/ kDa (g·mol⁻¹)	Number of subunits	pI	A280 (mg/ml)
IgA, human	162	4	n.a.	1.34
IgE, human	190	4	n.a.	1.53
IgG, goat	144	4	n.a.	1.30
IgG, human	153	4	6.6 - 7.2	1.38
Subclass IgG1, human	146	4	8.6 ± 0.4	n.a.
Subclass IgG2, human	146	4	7.4 ± 0.6	n.a.
Subclass IgG3, human	170	4	8.3 ± 0.7	n.a.
Subclass IgG4, human	146	4	7.2 ± 0.8	n.a.
IgG, mouse	155	4	∼ 7.0	1.40
IgG, rabbit	150	4	n.a	1.36
IgM, human	950	20	5.5 - 7.4	1.33

*polyclonal antibodies do not have a set isoelectric point as they are a mixture of various classes

Average serum concentration of human IgGs (mg/ml):

IgG1: 8
IgG2: 4
IgG3: 0.8
IgG4: 0.4

Human immunoglobulins are composed of 82-96% proteins and 4-18% carbohydrates

Mouse immunoglobulin subclasses: IgG1, IgG2a, IgG2b, IgG2c, IgG3
Rabbit immunoglobulin subclass: IgG (only one)
Rat immunoglobulin subclasses: IgG1, IgG2a, IgG2b, IgG2c

Technical information

Technical information > Molecular weight and isoelectric point of various animal immunoglobulins

Antibodies to unconjugated human immunoglobulins Immunoglobulins from various species in different purity grades

Antibodies to unconjugated human immunoglobulins

Immunoglobulins from various species in different purity grades