PIP2-7 | plasma membrane aquaporin (C-terminal)

336 €

AS12 2110 | clonality: polyclonal | host: rabbit | reactivity: L. sativa, P. sativum, S. lycopersicum, Z. mays

PRODUCT INFORMATION IN PDF

product information

background

Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a

immunogen

KLH-conjugated synthetic peptide derived from Zea mays PIP2-7 C-terminal, Q9ATM4

antibody format

rabbit

polyclonal,

serum

quantity

100 µl

storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

AS09 488 | PIP2;1 | aquaporin PIP2;1

AS09 490 | PIP2;2 | plasma membrane aquaporin 2b

AS09 491 | PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3

additional information

Protocol for isolation of plant plasma membrane proteins can be found here.

This antibody has a potential to work in immunolocalization studies, as it is recognizing C-terminal part of the sequence.

application information
recommended dilution		1: 3000 with standard ECL (WB)
expected \| apparent MW		30.7 \| 30 kDa (Zea mays)
confirmed reactivity		Lactuca sativa, Pisum sativum, Solanum lycopersicum, Zea mays
predicted reactivity		dictos including: Arabidopsis thaliana, Brassica oleracea, Glycine max, Medicago trunculata, Nicotiana tabacum, Spinacia oleracea, Solanum tuberosum, Vitis vinifera,monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, trees: Picea mariana, Populus trichocarpa
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		detection pattern consists of di and monomer of PIP2-7
selected references		to be added when available, antibody released in May 2012.

application example

10 µg of total protein from Zea mays roots (1), Phaseolus vulgaris leaves (2) or roots (3) extracted with a mixture of 250 mM sorbitol, 50 mM Tris–HCl (pH 8), 2 mM EDTA, and protease inhibitors [1 mM phenylmethylsulfonyl Xuoride, 1 mg ml^-1 each of leupeptin, aprotinin, antipain, chymostatin, and pepstatin were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk in TBS-T for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3.000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed four times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:30 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 60 seconds.

Courtesy of Dr. Ricardo Aroca, CSIC, Spain

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