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GAI | DELLA protein GAI

265 €
Buy 2 items of this product for 198 €/each
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AS11 1631 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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Item No:
AS11 1631

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product information
Background

GAI is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Less sensitive to GA. Synonymes:GRAS family protein 3, AtGRAS-3, Gibberelic acid-insensitive mutant protein, Restoration of growth on ammonia protein 2.

Immunogen

KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana GAI protein sequence UniProt: Q9LQT8, TAIR: At1g14920

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 25 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS13 2638 | anti-GID2 | F-BOX protein GID2 (SLEEPY 1), rabbit antibody
AS11 1630 | anti-RGA | DELLA protein RGA, rabbit antibody
AS11 1802 | anti-RGA-like protein | DELLA protein RGL1, rabbit antibody
AS11 1803 | anti-RGA-like protein 2 | DELLA protein RGL2, rabbit antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB) on transfected Arabidopsis thaliana protoplasts
Expected | apparent MW

58.9 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in

Solanum lycopersicum

Additional information
Selected references Shahnejat-Bushehri et al. (2016). Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. NATURE PLANTS 2: Article number: 16013, 2016.

Application example

western blot using anti-GAI antibodies
20 µg of total protein from Arabidopsis thaliana Columbia-0 plants grown on agar plates (MS 1 % sucrose) in LD conditions (16 h light, 8 h dark). Protein extraction was done using TRIZOL protocol on samples taken every 4 hours from the time point lights were switched on (Zeitgeber Time 0). Marker was 10 µl of BLUEstain™ Protein ladder, 11-254 kDa (GoldBio). Samples were separated on 12 % SDS-PAGE run at 200 V on Mini-Protean and blotted 1h to nitrocellulose. Blots were blocked with TBST (TBS+0.1 % Tween, 5 % non-fat, dry milk) for over night at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:00 000 in for 1h at RT with agitation. The blot was washed as above and developed with BIO-RAF Chemi-doc. Exposure time was 5 minutes.

Courtesy of Dr Federico Valverde, CSIC – University of Seville, Spain

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