2b protein [Tomato aspermy virus]
AS16 3982 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Tomato aspermy virus
|Info:||More information||Product suggestions||Add review|
1: 5000 (WB)
|Expected | apparent MW||
11.2 | 17 kDa
2b protein [Tomato aspermy virus].
|Not reactive in||No confirmed exceptions from predicted reactivity known at the moment.|
|Selected references||To be added when available, antibody released in September 2016.|
The indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions (GE Healthcare: RPN2236). Exposure time was 20 seconds.
Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA
||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at email@example.com