ACC | 1-Aminocyclopropane-1-carboxylic acid

350 €

AS11 1800   | clonality: polyclonal  |  host: rabbit  |  reactivity:ACC


33 st
Item No:
AS11 1800

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product information

ACC (1-Aminocyclopropane-1-carboxylic acid)  plays an important role in the biosynthesis of the plant hormone ethylene. It is synthesized by the enzyme ACC synthase ( EC from methionine and converted to ethylene by ACC oxidase (EC


conjugated ACC

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications immunogold (IG)
Related products

collection of antibodies against plant hormones

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 100 (IG)

Expected | apparent MW
Confirmed reactivity

ACC | 1-Aminocyclopropane-1-carboxylic acid

Predicted reactivity ACC | 1-Aminocyclopropane-1-carboxylic acid
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information details of used immunogold protocol are provided under an image.
Selected references

to be added when available, antibody released in December 2012.

application example

immunogold localization of ACC in plant tissue

Image shows gold particle density in Arabidopsis thaliana Col-0 after immunogold labeling of ACC in the transmission electron microscope. Gold particles could be detected in chloroplasts (C), mitochondria (M), peroxisomes (Px), and the dense cytosol. No or very few gold particles bound to ACC could be detected in vacuoles (V), intercellular spaces (IS) and cell walls (CW). Bar=1µm.

pre-immune serum control for anti-ACC antibody

Image shows leaf cell of Arabidopsis thaliana Col-0 treated with preimmune serum instead of the primary antibody against ACC in the transmission electron microscope. No or only very few gold particles were present on the sections. (C) chloroplast, (M) mitochondria, (Px) peroxisome. Bar=1µm.

Sample fixation: Samples for cytohistochemical analysis were fixed for 90 minutes in 2.5% paraformaldehyde/0.5% glutardialdehyde in 0.06M Sørensen phosphate buffer (pH 7.2). Samples were rinsed 4 times for 10 minutes in phosphate buffer then dehydrated with increasing concentrations of acetone (50%, 70% and 90% 20 min for each step). The samples were then transferred to increasing concentrations of LR-White resin (30%, 60%, 100%; London Resin Company Ltd., Berkshire, UK) mixed with acetone (90%) for at least 3 h at each step at RT. Finally, the samples were embedded in pure, fresh LR-White resin and polymerized at 50°C for 48 h in small plastic cups under anaerobic conditions.

Immunogold labeling for electron microscopy: Block ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2). Then treat the sections with the primary antibody diluted 1:100 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm; British BioCell International, Cardiff, UK) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.

Courtesy of Dr. Bernd Zechmann, Graz University, Austria

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