ACCase subunit beta | Acetyl-coenzyme A, carboxylase (subunit beta)

350 €

AS15 2880  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana


23 st
Item No:
AS15 2880

Info: Product suggestions Add review
product information

ACCase subunit beta (acetyl-coenzyme A, carboxylase (subunit beta)) is a component of the acetyl coenzyme A carboxylase (ACC) complex. It is catalyzing carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT).


Recombinant ACC from Arabidopsis thaliana UniProt: P56765, TAIR:ATCG00500

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS15 2881 | anti-Biotin carboxyl carrier subunit of chloroplast ACCase, rabbit antibody

Secondary antibodies

Additional information
application information
Recommended dilution

1:2500 (WB)

Expected | apparent MW

55.63 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Aethionema grandiflorum, Barbarea verna, Brassica napus, Capsella bursa pastoris, Carica papaya, Cardamine impatiens, Crucihimalaya wallichii, Draba nemorosa, Lepidium virginicum, Lobularia maritima, Nasturtium officinale, Olimarabidopsis pumila, Pachycladon enysii, Raphanus sativus, Theobroma cacao
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references to be added when available antibody released in June 2015

application example

Various amounts of recombinant AccD were separated on 12 % SDS-PAGE  and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

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