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Acetylated lysine (100 ĩl)

383 €

AS10 707-100  | clonality: monoclonal  |  host: mouse  |  reactivity: acetylated lysine

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AS10 707-100

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product information
Background

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). 

Immunogen

acetylated KLH

Host Mouse
Clonality Monoclonal
Clone IgG1 (clone 7F8)
Purity
Format Liquid
Quantity 100 ĩl
Reconstitution
Storage

store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Immunocytochmistry/Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Related products

AS15 3018 | anti-Acetylated Lysine, rabbit antibodies

AS10 707-100 | anti-Acetylated lysine (100 µl), mouse antibody

AS15 3019 | anti-Acetylated Lysine, APL conjugated, rabbit antibody
AS15 3020 | anti-Acetylated Lysine, FITC conjugated, rabbit antibody

Secondary antibodies

Additional information

protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml

antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.

application information
Recommended dilution

1: 100 (IHC), 1: 1000 (WB), for other assays optimal dilution has to be determined by end user

Expected | apparent MW
Confirmed reactivity

bovine, avian

Predicted reactivity

higher plants

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system

Selected references Vigushin & Coombes (2004). Targeted histone deacetylase inhibition for cancer therapy. Curr. Cancer Drug Targets 4: 205-218.


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