ACT | Actin (monoclonal)

335 €

AS16 3141  | clonality: monoclonal  |  host: mouse  |  reactivity: Arabidopsis thaliana, Dictyostelium discoideum


2 st
Item No:
AS16 3141

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product information
Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Immunogen Full length recombinant Arabidopsis thaliana Actin-8 (ACT8)  expressed in E.coli, Uniprot: Q96293
Host Mouse
Clonality Monoclonal
Clone IgG2b, clone mAbGPa (10-B3)
Quantity 100 ĩg

Store at -20°C.

Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Immunohistochemistry (IHC), Western blot (WB)
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AS13 2640 | anti-ACT | actin, rabbit antibody
AS10 681
| Anti-tubulin beta chain, rabbit antibody
AS10 680 | Anti-tubulin alpha chain, rabbit antibody

AS10 702 | Actin-11, mouse monoclonal antibody
AS16 3140 | ACT2,8,11 | Actin 1, 8, 11, mouse monoclonal antibody
AS16 3139 | ACT1,3,4,12 | Actin 1, 3, 4, 12, mouse monoclonal antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

This antibody is a total IgG fraction purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin

application information
Recommended dilution

1-2 ug/ml (WB)

Expected | apparent MW

45 | 45 kDa

Confirmed reactivity

Arabidopsis thaliana, Dictyostelium discoideum

Predicted reactivity Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Camelina sativa, Camellia lipoensis, Cucumis sativus, Dendrocalamus latiflorus, Dionaea muscipula, Gossypium sp., Eucalyptus grandis, Euphorbia lathyris, Gynura bicolor, Ficus microcarpa, Haloxylon ammodendron, Helianthus annuus, Hevea brasiliensis, Lilium regale, Medicago sativa, Mimosa pudica, Nitraria sibirica, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Oxytropis ochrocephala, Paeonia lactiflora, Panax notoginseng, Populus trichocarpa, Prunus avium, Ricinus communis, Striga asiatica, Theobroma cacao, Torenia fournieri, Trifolium pratense, Ziziphus jujuba
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281
Kandasamy, M.K. et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54
Kandasamy, M.K. et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-91.

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