ACT 1,3,4,12 | Actin 1, 3, 4, 12

335 €

AS16 3139  | clonality: monoclonal  |  host: mouse  |  reactivity: Arabidopsis thaliana


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Item No:
AS16 3139

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product information
Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Immunogen Full length recombinant Arabidopsis thaliana Actin-1 (ACT1) expressed in E.coli, Uniprot: P0CJ46
Host Mouse
Clonality Monoclonal
Clone IgG1, clone mAB45a (5-15 H7C5)
Quantity 100 ĩg

Store at -20°C.

Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
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AS16 3140 | ACT2,8,11 | Actin 1, 8, 11, mouse monoclonal antibody
AS16 3141 | ACT | Actin (monoclonal), mouse monoclonal antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

This antibody is a total IgG fraction purified on a protein G column. It recognizes the epitope Asn79 of actins ACT1, 3, 4, 12.

application information
Recommended dilution

1-2 ug/ml (WB)

Expected | apparent MW

45 | 45 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity C. sinensis, C. seticuspe, F. boreale, C. maxima, C. sativus, G. aurea, G. soja, G. arboreum, G. hirsutum, L. usitatissimum, L. cubeba, M. truncatula, M. indica, M. alba, N. tazetta var. chinensis, P.vulgaris, P. grandiflorus, P. tomentosa, P. trichocarpa, P. vulgaris, P. avium, R. communis, S. tuberosum, S. asiatica, T. cacao
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information Exact working dilution needs to be determined by end user.
Selected references Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281
Kandasamy, M.K. et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54
Kandasamy, M.K. et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-91.

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