AGB1 | Guanine nucleotide-binding protein beta 1

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AS16 3937 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


29 st
Item No:
AS16 3937

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product information

AGB1 belongs to a family of proteins involved in signal transduction. They are acting like molecular switches when transmitting signals from the outside to the inside of cells.


KLH-conjugated peptide derived from Arabidopsis thaliana AGB1 sequence, UniProt: P49177 TAIR: At4g34460

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AGB1/AGG1 | Guanine nucleotide-binding protein subunit beta 1 and gamma 1
GPA1 | Guanine nucleotide-binding protein subunit alpha 1
GPA1 | Guanine nucleotide-binding protein subunit alpha 1

RACK1 | Receptor for activated C kinase 1
RACK1A | Receptor for activated C kinase 1A
other antibodies to signal transduction pathway components

Plant protein extraction buffer

Secondary antibodies

Additional information

To be added when available

application information
Recommended dilution 1 : 1000
Expected | apparent MW 41 kDa | 35 kDa
Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Arabidopsis thaliana, Brasica sp., Cajanus cajan, Camelina sativa, Capsella rubella, Eutrema sp., Cicer arietinum, Gossypium sp., Medicago truncatula, Morus sp., Cajanus cajan, Pisum sativum, Sesamum indicum, Solanum sp., Tarenaya hassleriana, Theobroma cacao, Trifolium subterraneum
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

To be added when available

Selected references

To be added when available.

Application example
Western blot using anti-AGB1 antibody
The samples extracted either directly with SDS loading buffer (50mM Tris-HCl pH6.8, 100mM DTT, 2%SDS, 10% glycerol, 0.025% bromophenol blue) and then denatured at 95°C for 5 min, or first extracted with CE buffer (250mM sucrose, 100mM HEPES-KOH pH 7.5, 5% glycerol, 1mM Na2MoO4 x 2H2O, 25mM NaF, 10mM EDTA, 1mM DTT, 0.5%Triton X-100,  protease inhibitor cocktail) and then denatured with SDS loading buffer at 70°C for 5 min. 10 µl of proteins were loaded into each well and separated on 10% SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with Tris-buffered saline containing 0.05% Tween-20 (TBS-T)  and 5% skimmed milk powder for 1h at room temperature (RT) with agitation. The blot was incubated in the primary antibodies indicated at a dilution of 1: 5 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 15 min in TBS-T with milk powder at RT with agitation. Blot was incubated in secondary antibody (Goat-Anti-Rabbit AP conjugate, Sigma) diluted 1:5000  for 2h at RT with agitation. The blot was washed as above with TBS-T without milk powder, equilibrated in AP buffer (100mM TRIS pH=9.5, 100mM NaCl, 50mM MgCl2) and then developed with BioRad Immunstar AP substrate, before exposure to a CEA RP NEW film for 20 s.

Courtesy of Dr. Elena Petusching, Georg-August-University Goettingen, Germany

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