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AGB1 | Guanine nucleotide-binding protein beta 1

265 €
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AS16 3937 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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AS16 3937

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product information
Background

AGB1 belongs to a family of proteins involved in signal transduction. They are acting like molecular switches when transmitting signals from the outside to the inside of cells.

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana AGB1 sequence, UniProt: P49177 TAIR: At4g34460

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products AS16 3938 | Anti-AGB1/AGG1 | Guanine nucleotide-binding protein subunit beta 1 and gamma 1, rabbit antibodies
AS12 2370 | Anti-GPA1 | Guanine nucleotide-binding protein subunit alpha 1, rabbit antibodies
AS16 3940 | Anti-RACK1 | Receptor for activated C kinase 1, rabbit antibodies
AS11 1810 | Anti-RACK1A | Receptor for activated C kinase 1A, rabbit antibodies

Collection of antibodies to signal transduction pathway components

Plant protein extraction buffer
Secondary antibodies
Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW 41 kDa | 35 (37 kDa in 10 % gel) kDa
Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brasica sp., Cajanus cajan, Camelina sativa, Capsella rubella, Eutrema sp., Cicer arietinum, Gossypium sp., Medicago truncatula, Morus sp., Cajanus cajan, Pisum sativum, Sesamum indicum, Solanum sp., Tarenaya hassleriana, Theobroma cacao, Trifolium subterraneum
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information 10 % gel is recommended to use for a better protein resolution.
Selected references

To be added when available, antibody released in March 2017.


Application example

Western blot using anti-AGB1 antibodies

10 µl of protein samples from Arabidopsis thaliana were separated on SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with Tris-buffered saline containing 0.05% Tween-20 (TBS-T) and 5% skimmed milk powder for 1h at room temperature (RT) with agitation. The blot was incubated in the primary antibodies indicated at a dilution of 1: 5000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 15 min in TBS-T with milk powder at RT with agitation. The blot was incubated in secondary antibody (Goat-Anti-Rabbit AP conjugate, Sigma) diluted 1:5000 for 2h at RT with agitation. The blot was washed as above with TBS-T without milk powder, equilibrated in AP buffer (100mM TRIS pH=9.5, 100mM NaCl, 50mM MgCl2) and then developed with BioRad Immunstar AP substrate and imaged with a BioRad Chemi Doc Touch system. Exposure time was:  10 minutes.


Total protein from the indicated Arabidopsis thaliana lines was extracted with extraction buffer CE ( 250mM sucrose, 100mM HEPES-KOH pH 7.5, 5% glycerol, 1mM Na2MoO4 x 2H2O, 25mM NaF, 10mM EDTA, 1mM DTT, 0.5%Triton X-100, protease inhibitor cocktail). Protein concentration was measured with a Bradford assay and adjusted to 1mg/ml. Samples were denatured with SDS loading dye (50mM Tris-HCl pH6.8, 100mM DTT, 2%SDS, 10% glycerol, 0.025% bromophenol blue) at 70°C for 2-5 min.

Courtesy of Dr. Elena Petusching, Georg-August-University Goettingen, Germany


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