AGO6 | Argonaute 6
AS10 672 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1: 1000 (WB)
|Expected | apparent MW||
116.4 | 99 kDa
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
|Additional information||AGO6 expression is cell specific. Seedlings can be used as a negative control.|
Havecker, E.R., Wallbridge, L.M., Hardcastle, T.J., Bush, M.S., Kelly, K.A., Dunn, R.M., Schwach, F., and Baulcombe, D.C. The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. manuscript revisions submitted, awaiting acceptance.
360 µg/well of Arabidopsis thaliana protein extracted from leaf using TCA-protein precipitation and saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (DayLight 800) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.
Courtesy of Dr. Betty Chung and Pawel Baster, University of Cambridge, United Kingdom
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