AGO6 | Argonaute 6

371 €

AS10 672 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


12 st
Item No:
AS10 672

Info: Product suggestions Add review
product information

AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).


KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940 

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4. 
Format Lyophilized
Quantity 100 ĩg
Reconstitution For reconstitution add 100 ĩg of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibody
AS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) 
AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated)
AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) 
AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibody
AS09 527P | AGO1 | argonaute 1 | Blocking peptide
AS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibody
AS09 617 | Anti-AGO4 argonaute 4, rabbit antibody
AS10 671 | Anti-AGO5 | argonaute 5, rabbit antibody
AS10 673 | Anti-AGO9 | argonaute 9, rabbit antibody
AS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibody

collection of antibodies to micro RNA

Plant protein extraction buffer

Secondary antibodies

Additional information

There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used.

AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein was run on a 6% denaturing gel to obtain good separation.

If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.

TCA acetone total protein precipitation method

application information
Recommended dilution

1: 1000 (WB)

Expected | apparent MW

116.4 | 99 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information AGO6 expression is cell specific. Seedlings can be used as a negative control.
Selected references

Havecker, E.R., Wallbridge, L.M., Hardcastle, T.J., Bush, M.S., Kelly, K.A., Dunn, R.M., Schwach, F., and Baulcombe, D.C. The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. manuscript revisions submitted, awaiting acceptance.

Application example

western blot using anti-AGO6 antibodies'

360 µg/well of Arabidopsis thaliana protein extracted from leaf using TCA-protein precipitation and saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (DayLight 800) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner. 

Courtesy of Dr. Betty Chung and Pawel Baster, University of Cambridge, United Kingdom

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at