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AHB2 | Hemoglobin 2

265 €
Buy 2 items of this product for 198 €/each
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AS13 2745  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

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Item No:
AS13 2745

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product information
Background

AHB2 (Arabidopsis hemoglobin2) belongs to plant globin family and is a heme, iron and oxygen binding protein. Expressed in rosette leaves and young roots and induced by variety of environmental stresses. Synonymes:AHB2, ARABIDOPSIS HEMOGLOBIN 2, ARATH GLB2, ATGLB2, GLB2, HAEMOGLOBIN 2, HB2, HEMOGLOBIN 2, NON-SYMBIOTIC HAEMOGLOBIN 2, NSHB2.

Immunogen

recombiant Arabidopsis thaliana AHB2 protein, UniProt:O24521, TAIR: AT3G10520

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

17.9 | 17.9 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Beta vulgaris, Brassica napus, Gossypium hirsutum, Solanum lycopersicum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in August 2014.


application example

western blot using anti-AHB2 antibodie

Reactivity of AHB2 antibodies to recombinant AHB1 and AHB2
ng of recombinant Arabidopsis thaliana AHB1 and AHB2 are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.

Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA  

western blot using anti-AHB2 antibodies

AHB2 expression in stage 1-15 flowers
Indicated protein amounts of Arabidopsis thaliana extracts are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.

Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA  



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