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Akt/PKB | Protein kinase B, (pT308)

585 €

AS16 4020 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human, Mouse, Rat

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AS16 4020

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product information
Background AKT/Protein Kinase B is a closely related serine/threonine-protein kinases called the AKT kinase. This protein regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Akt is phosphorylated on thr 308 by PDK1 and on ser 473 by PDK2.
Immunogen Synthetic phosphopeptide derived from the human VEGFR2 protein which contains Y1054. UniProt: Human: P31749, Mouse: P31750, Rat: P47196
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum.
Format Liquid
Quantity For 10 Miniblots.
Reconstitution
Storage Store at -20°C.
Tested applications Westerm blot (WB)
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Additional information Provided in phosphate buffered saline, pH 7.3, with 1.0 mg/ml BSA and 0.05% sodium azide.

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Akt protein. The final product is generated by affinity chromatography using an Akt-derived peptide that is phosphorylated at threonine 308. 
application information
Recommended dilution 0.35-1.0 µg/mL (WB). At 0.50 µg/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed. The optimal antibody concentration should be determinedby the end user.
Expected | apparent MW
Confirmed reactivity Human, Mouse, Rat
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.
Additional information
Selected references To be added when available, antibody released in November 2016.


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