FOX1 | plasma membrane ferroxidase FLP1
AS06 120 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii
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1: 1000 - 1: 5000 with standard ECL (WB),
|Expected | apparent MW||
122 | 120 kDa
|Confirmed reactivity||Chlamydomonas reinhardtii|
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.
|Selected references||Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
LaFontaine et al. (2002) Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell 1(5):736-757
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