HSP70B | stromal alfa-HSP70

320 €

AS06 175 | clonality: polyclonal | host: rabbit | reactivity: Ch. reinhardtii, D. subspicatus, P. patens

PRODUCT INFORMATION IN PDF

Info:

Product suggestions

Add review

product information

background

HSP70B is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas reinhardtii chloroplasts. It interacts with HSP90C, CGE1, CDJ2, and VIPP1.

immunogen

mature HSP70B protein expressed with N- and C-terminal hexahistidine tags in E. coli, purified with Ni-NTA

antibody format

rabbit

polyclonal

serum

lyophilized

quantity

100 µl

for reconstitution add 100 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB) immunoprecipitation (IP)

related products

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

additional information

to be added when available

application information
recommended dilution		1:10 000 with standard ECL (WB)
expected \| apparent MW		71.9 kDa
confirmed reactivity		Chlamydomonas reinhardtii, Desmodesmus subspicatus, Physcomitrella patens
predicted reactivity		Dunaliella salina, cyanobacteria
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		to be added when available
selected references		Diaz-Troya et al. (2011). Inhibition of protein synthesis by TOR inactivation revealed a conserved regulatory mechanism of the BiP chaperone in Chlamydomonas. Plant Physiol, in press. Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source) Tukaj and Tukaj (2010). Distinct chemical contaminants induce the synthesis of Hsp70 proteins in green microalgae Desmodesmus subspicatus: Heat pretreatment increases cadmium resistance. Journal of Thermal Biology 35 (5):239-244.

||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com