AMY | Alpha-amylase

345 €

AS10 712  | clonality: polyclonal  |  host: rabbit  |  reactivity: Oryza sativa


31 st
Item No:
AS10 712

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product information

α-Amylases are hydrolytic enzymes responsible for the mobilization of the starch into metabolizable sugars. This process provides the energy for the growth of roots and shoots and is crucial during germination of cereal seeds.These enzymes are coded by a multigene family and even thought other amylolytic enzyme participate in the process of starch breakdown, the contribution of α-amylase is the prerequisite for the initiation of this process.


KLH-conjugated synthetic peptide derived from known Oryza sativa P17654

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS10 716 | Ramy 3D | Alpha-amylase isozyme 3D

Plant protein extraction buffer

Secondary antibodies

Additional information

antibody will detect all alpha amylase isoforms from rice, barley and other cereals

application information
Recommended dilution

1 : 5000 with standard ECL (WB)

Expected | apparent MW

43-48 kDa (depending upon the isoform)

Confirmed reactivity

Oryza sativa, Panicum virgatum

Predicted reactivity

cereals, Kalanchoe laxiflora

Not reactive in

Pinus strobus

Additional information

to be added when available

Selected references

to be added when available, antibody released in December 2010.

application examples

alpha-Ramy detection in rice using western blot

25 µg of total protein from Oryza sativa seedlings (from 1 day to 5 days of imbibition on filter paper at 28°C) extracted with an SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2% SDS, 1,5% Sucrose) were separated on XT CRITERION 10%Bis-Tris (BioRad) SDS-PAGE and blotted 1h to PVDF. Blot was blocked immediately in milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a diluition of 1: 5,000 in milk in TBS-T for 3h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated, AS09 602) diluited 1:20 000 in milk in TBS-T for 50 min at RT and then washed as above and developed for 3 min with standard ECL. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Exposure time was 30 min. The arrow indicates alpha-amylase (between 47kDa and 50KDa as expected).

Courtesy Valeria Banti and prof. Pierodomenico Perata, PlantLab, Scuola Superiore Sant'Anna

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