SOD1 aa 80-96 | superoxide dismutase 1, soluble
AS13 2645 | clonality: monoclonal | host: mouse | reactivity: human
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1:1000-1:10 000 (ELISA), 1:1000 with standard ECL (WB)
|Expected | apparent MW||
|Predicted reactivity||bovine,chimpanzee, dog, goat, guinea pig, mouse, pig, rabbit, rat, sheep, fission yeast|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
to be added when available
to be added when available, antibody released in March 2013.
100,10 and 1 ng of recombinant human SOD1 were separated by 4-20 % SDS-PAGE and transferred electrophoretically (25V, 10 min) onto PVDF membrane. Non-specific binding sites were blocked by incubating membrane with 5 % dry milk in PBS, 0.1 % Tween 20 for 1h at room temperature (RT) with agitation. The membrane was thereafter incubated with the primary antibody SOD1 aa 58-72 at a dilution of 1: 1 000 for 3h at RT with agitation. The antibody solution was decanted and the membrane was rinsed 3 times for 5 min in PBS-T (0.05 %) at RT with agitation. The membrane was then incubated with the secondary antibody (Rabbit Anti-Mouse IgG – HRP conjugated (DAKO) at a 1:1 000 dilution) for 1h at RT with agitation. The membrane was washed as above and developed for 5 min with Amersham ECL prime western blotting detection reagent according to the manufacturer’s instructions (GE Healthcare). Exposure time was 15 s.
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