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product information
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| background |
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Proteasome-dependent degradation serves an essential role in the removal of a wide variety of key nuclear and cytosolic proteins. Substrates are targeted for proteolysis by the ubiquitin pathway before being degraded by the 26 S proteasome. The subunit of the proteasome, S5a, was identified to bind to polyubiquitin in vitro and thus proposed to act as a substrate recognition component. |
| immunogen |
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Drosophila mealnogaster 26S Proteasome subunit S5a also known as Rpn10, p54 |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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100 µl, |
for reconstitution add 100 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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ASA01 019 | human 26S proteasome subunit |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1:2000 (WB) |
| expected | apparent MW |
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42.6 | 54 kDa Drosophila, 40 kDa Arabidopsis |
| confirmed reactivity |
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Arabidopsis thaliana, Drosophila melanogaster |
| predicted reactivity |
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bovine, mouse, pig, salmon, rat, |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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n.a. |
| selected references |
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Lundgren et al. (2003). Use of RNA interference and complementation to study the function of the Drosophila and human 26S proteasome subunit S13. Mol Cel Biol 23:5320-5330. |
application example 
3 µg of total protein from Arabidopis thaliana leaves extracted with CelLytic P (Sigma) were separated on 10 % SDS-PAGE and blotted overnight at 30V to a nitrocellulose membrane. Blots were blocked with 1% western blocking reagent (Roche) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (DS5a) at a dilution of 1:2000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, and then washed twice for 10 min with TBS-T at RT with agitation. Subsequently, the blot was washed twice with 0,5% western blocking reagent for 10 min each. Blot was incubated in secondary antibody (Goat anti-rabbit IgG (H&L) HRP conjugated, AS09 602 from Agrisera) diluted to 1:50 000 in 0,5% western blocking reagent for 1h at RT with agitation. The blot was washed 4 times with TBS-T buffer. Detection of HRP was performed with ECL according to the manufacturer's instructions (GE healthcare). Exposure time was 2 seconds. Position of protein on gel corresponds to expected size - 40 kDa. Courtesy Dr. Jozefus Schippers, Max Planck Institute, Germany
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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