Animal cell antibodies / Bacterial, insect and fungal

product information

Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

western blot (WB)

n.a.

to be added when available

application information

1: 5000 (WB)

48 kDa

Pseudomonas aeruginosa

Pseudomonas aeruginosa only

no confirmed exceptions from predicted reactivity known in the moment

not available at the moment

Henriksson et al. (2002). A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo. Eur.J Biochem. 20:4921-4929.   Yasmin et al. (2006). Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity. FEBS J. 3:638-646.