HDEL | endoplasmic reticulum retention signal (100 µg)

345 €

AS10 683 | clonality: monoclonal | host: mouse | reactivity:Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata, S. cerevisiae, D. melanogaster

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background

HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

immunogen

synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

antibody format

mouse

monoclonal

IgG2B (clone 2E7)

liquid

quantity

100 µg

storage

store at -20°C for 1 year; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), immunofluorescence (IF), immunogold (IG)

related products

AS10 683-25 | HDEL | endoplasmic reticulum retention signal mouse antibody, 25 µg

AS13 2665 | KDEL | endoplasmic reticulum retention signal (50 µg), rabbit antibody

collection of antibodies for endomembrane proteins

additional information

protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml

application information
recommended dilution		1: 100 - 1. 1000 (WB), 1. 50- 1: 500 (IF)
expected \| apparent MW		78 kDa
confirmed reactivity		Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata, S. cerevisiae, D. melanogaster
predicted reactivity		not analyzed
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992
selected references		Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697. Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.

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