HDEL | endoplasmic reticulum retention signal (25 µg)

173 €

AS10 683-25 | clonality: monoclonal | host: mouse | reactivity:Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata, S. cerevisiae, D. melanogaster

PRODUCT INFORMATION IN PDF

Info:

Product suggestions

Add review

product information

background

HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

immunogen

synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

antibody format

mouse

monoclonal

IgG2B (clone 2E7)

liquid

quantity

25 µg

storage

store at -20°C for 1 year; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), immunofluorescence (IF), immunogold (IG)

related products

AS10 683 | HDEL | endoplasmic reticulum retention signal mouse antibody, 100 µg

AS13 2665 | KDEL | endoplasmic reticulum retention signal (50 µg), rabbit antibody

collection of antibodies for endomembrane proteins

additional information

protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml

application information
recommended dilution		1: 100 - 1. 1000 (WB), 1. 50- 1: 500 (IF)
expected \| apparent MW		78 kDa
confirmed reactivity		Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata, S. cerevisiae, D. melanogaster
predicted reactivity		not analyzed
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992
selected references		Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697. Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.

||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com