Animal cell antibodies / Bacterial, insect and fungal

product information

Pseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an opportunistic pathogen of plants. P. aeruginosa bacterias are clinically important because they are resistant to most antibiotics and they are capable of surviving in conditions that few other organisms can tolerate. Pseudomonas is often encountered in hospital and clinical work because it is a major cause of hospital acquired (nosocomal) infections. Its main targets are immunocompromised individuals, burn victims, and individuals on respirators or with precipitation procedure followed by indwelling catheters. Additionally, these pathogens colonize the lungs of cystic fibrosis patients. P. aeruginosa is often identified by its pearlescent appearance and grape-like odor in vitro. Definitive clinical identification of P. aeruginosa includes identifying the production of both pyocyanin and fluorescein as well as its ability to grow at 42°C.

whole, formaldehyde killed, bacteria, strains PAO1 and Habs1

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Western blot (WB)

total IgY concentration is 13.2 mg/ml

the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation

application information

1: 1000 - 1: 5 000 with standard ECL (WB), 1: 1000 - 1: 5 000 (ELISA)

n. a.

Pseudomonas aeruginosa strains PAO1 and Habs1

n. a.

no confirmed exceptions from predicted reactivity known in the moment

reacts with P. aeruginosa strains PAO1 and Habs1; it will also crossreact with other P. aeruginosa strains in ELISA and immunoblotting

to be added when available