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product information
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| background |
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Saccharomyces cerevisiae replication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody
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| immunogen |
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RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa); overexpressed in E.coli and purified by chromatography; no affinity tags were added to any of three subunits |
| antibody format |
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rabbit; |
polyclonal; |
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serum; |
lyophilized |
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| quantity |
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100 µl |
- for reconstitution add 100 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB), Immunoprecipitation (IP) |
| related products |
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to be addedwhen available |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 20 000 with standard ECL (WB) |
| expected | apparent MW |
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70 + 30 + 14 kDa |
| confirmed reactivity |
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Saccharomyces cerevisiae |
| predicted reactivity |
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Saccharomyces cerevisiae |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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antibody was also successfully used in ChIP application; presented data are courtesy of M. Pool and Dr. H. van Attikum |
| selected references |
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to be added when available |
application example 
TCA precipitated protein extracts from a wild type yeast strain (S. cerevisiae) were separated on 10% gel and transferred to a PVDF membrane. Antibody was used in different dilutions: 1: 5000 (1); 1: 10 000 (2); 1: 20 000 (3); Besides the bands for RFA1 and RFA2 an unspecific band was detected at ~150 kDa.
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