Rnr3 | Ribonucleoside-diphosphate reductase large chain 2

345 €

AS09 574 | clonality: polyclonal | host: rabbit | reactivity: Saccharomyces cerevisiae

PRODUCT INFORMATION IN PDF

product information

background

Saccharomyces cerevisiae Rnr3 is catalyzing the biosynthesis of deoxyribonucelaotides. Alternative names: Ribonucleotide reductase large subunit 2, ribonucleotide reductase DNA damage-inducible regulatory subunit 2, ribonucleotide reductase R1 subunit 2

immunogen

KLH-conjugated synthetic peptide derived from Saccharomyces cerevisiae Rnr3 protein sequence P21672

antibody format

rabbit;

polyclonal;

affinity purified serum;

lyophilized

quantity

250 µg

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB)

related products

AS09 575 | anti-Rnr2 antibodies

AS09 576 | anti-Rnr1 antibodies

AS10 847 | anti-Sml1 | Suppressor of Mec1 lethality

additional information

recommended western blot protocol can be found here

application information
recommended dilution		1: 500-1:1000 with standard ECL (WB)
expected \| apparent MW		97.5 \| 98 kDa
confirmed reactivity		Saccharomyces cerevisiae
predicted reactivity		Saccharomyces cerevisiae
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		load per well was approx 3x10^6 cells (of a total extract)
selected references		Tsaponina et al. (2011). Ixr1 Is Required for the Expression of the Ribonucleotide Reductase Rnr1 and Maintenance of dNTP Pools. PLoS Genetics.

application example

western blot using anti-Rnr3 antibodies

10 μl total protein from 9.25 x 107 cells of Saccharomyces cerevisiae extracted with 20% TCA as described below were separated on 10% SDS-PAGE and blotted 1.5h (0.5 A) to a nitrocellulose membrane (Whatman PROTRAN BA 85, 0.45 μm). Blots were blocked with 5% non-fat dry milk in TBST for 1.5h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 200 overnight at 4C° with agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBST at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, (AS09 602) diluted to 1:50 000 for 1h at RT with agitation. The blot was washed as above and developed for 3 min with SuperSignal West Pico Stable Solution (1856135 Thermo Scientific) and SuperSignal West Pico Luminol/Enhancer Solution (1856136 Thermo Scientific) mixed 1:1 rate according to the manufacturers instructions. Exposure time was 10 min.

Courtesy Dr. Andrei Chabes, Umeå University, Sweden

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