HSP70/HSC70 | Heat shock protein 70
AS09 592 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Acanthamoeba castellanii (amoeba), Dictyostelium discoideum, frog-heart, Frog-skeletal muscle, Frog-liver, Caenorhabditis elegans, salmon (Salmo salar) , rainbow trout (Oncorhynchus mykiss), cow, chicken, pig, rat, seal, mummichog
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1:10 000 with ECL Advance (WB)
|Expected | apparent MW||
Arabidopsis thaliana, Acanthamoeba castellanii (amoeba), Dictyostelium discoideum, frog-heart, Frog-skeletal muscle, Frog-liver, Caenorhabditis elegans, salmon (Salmo salar) , rainbow trout (Oncorhynchus mykiss), cow, chicken, pig, rat, seal, mummichog
atlantic salmon (Salmo salar), brook trout (Salvelinus fontinalis)
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
not available at the moment
Chandra et al. (2012). Sustained high temperature increases the vitellogenin response to 17 alpha-ethynylestradiol in mummichog (Fundulus heteroclitus). Aquatic toxicology.
Courtesy Dr. Małgorzata Słocińska, UAM, Poland
5 µg of total protein from (1) cow muscle, (2) chicken muscle, (3) pig muscle, (4) rat liver, (5) salmon muscle , (6) seal muscle, (7) mummichog heat shock control, (8) mummichog heat shock post-24 hours, extracted with Protein Extration Buffer, PEB (AS08 300), were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (Agrisera anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.
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