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product information
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| background |
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Rat alpha-1-inhibitor 3 (A1i3) is a proteinase inhibitor in rat plasma. It belongs to the same superfamily as human alpha-2-macroglobulin (a2M). Alternative names: Alpha-1-inhibitor 3 variant II antibody, Alpha-1-inhibitor III antibody |
| immunogen |
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purified rat alpha-1-inhibitor 3 P14046 |
| antibody format |
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rabbit |
polyclonal |
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total IgG in PBS pH 7.4; |
lyophilized |
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| quantity |
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200 µg |
- for reconstitution add 200 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB) |
| related products |
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to be added when available |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 1000 with standard ECL (WB) |
| expected | apparent MW |
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164 | 180 kDa |
| confirmed reactivity |
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rat |
| predicted reactivity |
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rat |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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Kim et al. (2008) 2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter. Proteomics, 8(17):3632-44. |
application example - Western blot | Blotting with rabbit anti-rat alpha-1-inhibitor 3. Ten ml of rat plasma, diluted 200 times with PBS, and 20 ng purified rat alpha-1-inhibitor 3 were separated on a 9% polyacrylamide gel, transferred to a PVDF membrane, blocked, incubated 2 h at RT with anti-alpha-1-inhibitor 3 IgG, 1 mg/ml, washed and then incubated for 30 min with swine anti-rabbit IgG-HRP and washed. The membrane was developed using the ECL system. | | 
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com
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