ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

A1i3 | alpha-1-inhibitor 3

345 €

AS07 272  |  clonality: polyclonal  |  host: rabbit  |  reactivity: rat

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
7 st
Delivery:
Shipping:
Item No:
AS07 272

Info: Product suggestions Add review
product information
Background

Rat alpha-1-inhibitor 3 (A1i3) is a proteinase inhibitor in rat plasma. It belongs to the same superfamily as human alpha-2-macroglobulin (a2M). Alternative names: Alpha-1-inhibitor 3 variant II antibody, Alpha-1-inhibitor III antibody

Immunogen

Purified Rat alpha-1-inhibitor 3 P14046

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products
Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

164 | 180 kDa

Confirmed reactivity

rat

Predicted reactivity

rat

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references Kim et al. (2008) 2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter. Proteomics, 8(17):3632-44.

application example - Western blot

 

Experimental conditions:

Blotting with rabbit anti-rat alpha-1-inhibitor 3. Ten ml of rat plasma, diluted 200 times with PBS, and 20 ng purified rat alpha-1-inhibitor 3 were separated on a 9% polyacrylamide gel, transferred to a PVDF membrane, blocked, incubated 2 h at RT with anti-alpha-1-inhibitor 3 IgG, 1 mg/ml, washed and then incubated for 30 min with swine anti-rabbit IgG-HRP and washed. The membrane was developed using the ECL system.
 
 
http://www.agrisera.com/dokument/bibliotek/Image/AS07%20272%20western%20blot.jpg

 

 


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com