ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

AMBP | Alpha-1-microglobulin (clone BN11.10)

314 €

AS01 013 | clonality: monoclonal | host: mouse | reactivity: human, rat | Note: Limited stock

PRODUCT INFORMATION IN PDF

Availability:
0 st
Delivery:
Shipping:
Item No:
AS01 013

Info: Product suggestions Add review
product information
Background

Alpha-1-microglobulin is a 27-kDa glycoprotein with immunosuppressive properties. A gene AMBP in the lipocalin cluster encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Alpha-1-microglobulin is found in blood and in connective tissue in most organs. It is most abundant at interfaces between the cells of the body and the environment, such as in lungs, intestine, kidneys and placenta. Alpha 1-microglobulin is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function.

Immunogen

human and rat alpha-1-microglobulin, native, full length, purified from urine, UniProt: P02760 cleavege produt no. PRO_0000017886

Host Mouse
Clonality

monoclonal;

Clone

IgG2a;

Purity
Format

lyophilized

Quantity

100 µg

Reconstitution

- for reconstitution add 200 µl of sterile water

Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

Western blot (WB), ELISA (ELISA), immunoprecipitation (IP), radioimmunoassay (RIA), immunohistochemistry (IHC), affinity chromatography (ACH), immunoflourescence (IF)

Related products

AS01 014 | anti-rat alpha-1 microglobulin

Secondary antibodies

Additional information

antibody is purified and contains 0.5 mg/ml of mouse albumin in 150 mM NaCl, 20 mM Tris-HCl, pH 8 and 0.02% sodium azide;

This antibody is also reacting with the AMBP precursor.

application information
Recommended dilution

1: 2000 (WB), 0.2-2 mg/L (ELISA), 5 µg/µl (IP), 20 ng/ml (RIA), 1 µg/ml (IHC), 10 mg/1ml of gel (ACH), 5 µg/ml (IF)

Expected | apparent MW

27 kDa

Confirmed reactivity

human, guinea pig, rabbit, rat

Predicted reactivity

to be added when available

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

for usage on rabbit and guinea pig the antibody has to be applied in high concentrations.

The antibody has been used on frozen sections only, not paraffin. Suggested antigen retrieval is 5 min in microwave owen in citrate buffer, pH 6.

Selected references

Olsson et al. (2008). The lipocalin alpha1-microglobulin protects erythroid K562 cells against oxidative damage induced by heme and reactive oxygen species. Free Radic. Res. 42:725-732; Yokota et al. (2007) Absence of increased alpha1-microglobulin in IgA nephropathy proteinuria. Mol Cell Proteomics, 6:738-44.



||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com