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Abeta (1-42) | Amyloid-beta peptide 1-42

291 €

AS08 328  |  clonality: polyclonal  |  host: rabbit  |  reactivity: human

PRODUCT INFORMATION IN PDF

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Item No:
AS08 328

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product information
Background

Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of  the amyloid precursor protein P05067

Immunogen

synthetic peptide chosen from human Abeta (1-42) protein. Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Dot Blot (Dot), immunolocalization (IL), Western blot (WB)
Related products

Alzheimer | available antibodies for Alzheimer's disease

Additional information
application information
Recommended dilution

1:1000 (DB), 1:3000 (ELISA), 1-2 µg/ml (IL)

Expected | apparent MW

4.5 kDa

Confirmed reactivity

human

Predicted reactivity

bovine, chicken, dog, porcine, rabbit

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

the antibody can detect Abeta (1-42), Abeta (1-28) Abeta (1-20) and Abeta (1-17). This product exhibits a low reactivity to monomeric Abeta (1-42) as determined by SDS-PAGE and Western blotting.

Immunolocalization:
human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.

Selected references Lindhagen-Persson et al. (2010). Amyloid-β oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies. PLoS One. 2010 Nov 10;5(11):e13928. doi: 10.1371/journal.pone.0013928.

Dot blot experiment


• Membrane: Nitrocellulose
• Blocking buffer: 5% dry milk in PBS, 0.1% Tween 20
• Antibody dilution: 1:1000
• Secondary antibody: anti-rabbit (HRP)
• Detection: Enhanced Chemoluminescence (pico) 30s

*Abeta (1-42) consists of both monomeric and partly aggregated mtrl.


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