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PEN2 | gamma-secretase subunit PEN-2

323 €

AS06 154  | clonality: polyclonal  | host: rabbit  | reactivity: human, mouse, hamster

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AS06 154

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product information
Background

One characteristic feature of Alzheimer’s disease is the deposition of amyloid-beta peptide (Abeta) as amyloid plaques within specific regions of the human brain. Abeta is derived from the amyloid-beta peptide precursor protein (beta-APP) by the intramembranous cleavage activity of gamma-secretase. PEN-2 (Q9NZ42) is a critical component of PS1/gamma secretase (Farmery et al. 2003; Bergman et al. 2004).Alternative name:  presenilin enhancer protein 2

Immunogen

the antibody was raised against the N-terminal eleven residues of human PEN-2. PEN-2 is the smallest component of the multi-protein gamma-secretase complex. Chosen synthetic peptide has been conjugated to KLH

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Lyophilized
Quantity 100 ĩg
Reconstitution For reconstitution add 83.3 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

Alzheimer | available antibodies for Alzheimer's disease

Secondary antibodies

Additional information
application information
Recommended dilution

1:250 (WB)

Expected | apparent MW

15 kDa

Confirmed reactivity

human, mouse, hamster

Predicted reactivity

bovine, rat, Xenopus

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

approx. MW of the target protein is 15 kDa. Antibodies recognize also an unknown protein at around 40 kDa

Selected references

to be added when available


Western blot experiment example


Rat brain homogenate (15 µg). Overnight incubation of PVDF membrane with Pen-2 antibody (5 µg/ml) in PBS containing 1% BSA. Horseradish peroxidase-conjugated anti-rabbit antibody (0.5 µg/ml, GE Biosciences) was used for detection.


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