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product information
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| background |
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Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids and thyroid hormones, and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. |
| immunogen |
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full length native protein P02769 |
| antibody format |
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hen; |
polyclonal; |
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affinity purified IgY at 1 mg/ml in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2; |
liquid |
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| quantity |
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| storage |
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store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB), immunodifusion (ID) |
| related products |
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AS99 002 | anti-BSA rabbit antibody AS02 023 | anti-BSA hen antibody IMS06-035-327 | anti-BSA hen antibody, biotinylated |
| additional information |
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affinity purified IgY on bovine serum albumin agarose |
application information
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| recommended dilution |
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1: 5 000 with standard ECL (WB) |
| expected | apparent MW |
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67 kDa |
| confirmed reactivity |
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bovine |
| predicted reactivity |
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goat, sheep |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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only one precipitin arc in immunodiffusion against normal bovine serum, dilution 1:100, or bovine albumin. Only one precipitin arc in immunoelectrophoresis against normal bovine serum or bovine serum albumin. |
| selected references |
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to be added when available |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menu bar or contact us at support@agrisera.com
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