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ATG5 | Autophagy protein 5

350 €

AS13 2695 | clonality: polyclonal | host: rabbit | reactivity: human

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AS13 2695

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Background

FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Immunogen

A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B  has been used to enhance the immune response.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

After reconstitution keep aliquots at -20ºC for a higher stability, and at  4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Tested applications immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
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Additional information
application information
Recommended dilution 1 : 200-1 : 1000 (IF), (IHC), (WB)
Expected | apparent MW
Confirmed reactivity Human
Predicted reactivity
Not reactive in Arabidopsis thaliana
Additional information
Selected references

to be added when available


Application example




Confocal microscopy on stained ATG5 using Rabbit antibody to ATG5: whole serum (AS13 2695) in paraffin-embedded human brain section (midfrontal cortex, Alzheimer case). ATG5 appears green. Red staining is p25.







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