LRIG1 | Lig-1
AS06 148 | clonality: polyclonal | host: rabbit | reactivity: human, mice
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1 µg/ml (WB), 1: 100 (IL)
|Expected | apparent MW||
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
immunoblotting demonstrated LRIG1 protein in tissue lysates from normal human prostate, mammary epithelial cells, ileum, stomach, lung, and cerebral cortex
|Selected references||Wang et al. (2015). Loss of lrig1 leads to expansion of brunner glands followed by duodenal adenomas with gastric metaplasia. Am J Pathol. 2015 Apr;185(4):1123-34. doi: 10.1016/j.ajpath.2014.12.014.
Häggström et al. (2014). Potential upstream regulators of cannabinoid receptor 1 signaling in prostate cancer: A Bayesian network analysis of data from a tissue microarray. Prostate. 2014 Aug;74(11):1107-17. doi: 10.1002/pros.22827. Epub 2014 Jun 9.
5 µg of total protein from LRIG1-transfected COS-7 cells extracted with lysis buffer (100 mM Tris-HCl pH 7.5 1M, 300mM NaCl 5M, 2% Triton X-100) were separated on 3-8 % Tris acetate SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% dried milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at the indicated concentrations for 1h at RT with agitation.The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:5 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.
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