LRIG1 | Lig-1

441 €

AS06 148 | clonality: polyclonal | host: rabbit | reactivity: human, mice

product information

background

LRIG1 protein Q96JA1 is coded by a gene on a chromosome band 3p14.3, this region is known to be deleted in various human cancers. LRIG1 is considered to be a a tumour suppressor gene in humans. LRIG1 is an integral cell-surface membrane protein that is expressed by specific cells in various human tissues and that its 143-kDa form might be cleaved into 111-kDa and 32-kDa fragments. The LRIG1 protein may inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor.

immunogen

KLH-conjugated synthetic peptide; the peptide corresponds to part of the cytoplasmic tail (C-terminal part) of the protein

antibody format

rabbit;

polyclonal;

affinity purified IgG in PBS pH 7.4, BSA 1mg/ml;

lyophilized

quantity

100 µg

- for reconstitution add 90,9 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB), immunohistochemistry (IHC)

related products

AS06 156 | anti-LRIG1 hen antibody

additional information

Peptide used to elicit Lrig1 antibody is not conserved in Lrig3 protein.

application information
recommended dilution		1 µg/ml (WB), use at an assay dependent dilution (IHC)
expected \| apparent MW		143 kDa
confirmed reactivity		human, mice
predicted reactivity		human
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		immunoblotting demonstrated LRIG1 protein in tissue lysates from normal human prostate, mammary epithelial cells, ileum, stomach, lung, and cerebral cortex
selected references		Ledda et al. (2008). Lrig1 is an endogenous inhibitor of Ret receptor tyrosine kinase activation, downstream signaling, and biological responses to GDNF. J. Neruosci. 28(1):2196-07. Nilsson et al. (2003) LRIG1 protein in human cells and tissues. Cell and Tissue Research, 312(1): 65-71.

application information

western blot

western blot using anti-LRIG1 rabbit antibody

5 µg of total protein from LRIG1-transfected COS-7 cells extracted with lysis buffer (100 mM Tris-HCl pH 7.5 1M, 300mM NaCl 5M, 2% Triton X-100) were separated on 3-8 % Tris acetate SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% dried milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at the indicated concentrations for 1h at RT with agitation.The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:5 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.

5 µg of total protein from LRIG1-transfected COS-7 cells extracted with lysis buffer (100mM Tris-HCl pH 7.5 1M, 300mM NaCl 5M, 2% Triton X-100) were separated on 3-8 % Tris acetate SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% dried milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody [1ug/uL] at a dilution of 1:3500, 1:1600, 1:800 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:5 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was  30 seconds.

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