Animal cell antibodies / Human proteins

product information

mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.

recombinant human purified 6-His mPGES-1 O14684

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB) Immunohistochemistry (IHC)

AS03 031S | mPGES-1 glutathione dependent prostaglandin E synthase protein | positive control

in our hands this antibody works with fresh frozen sections not in paraffin embeded material in IHC

mPGES-1 antibody was characterized using spleen tissue from LPS-induced mPGES-1 knock-out and wild type mice. In spleen tissue from wt mice we observed strong positive staining in macrophage-like cells, while there was no staining for mPGES-1 in ko mice.

application information

1:10 000 on human and rat samples (WB) 1:6 000 on  mice samples and in sheep seminal vesicles (IHC)

17 kDa

human, mouse, rat, sheep

dog, hamster, horse

no confirmed exceptions from predicted reactivity known in the moment

antibody is only recognizing mPGES1 not mPGES2 or cPGES

Brenneis et al. (2011). Anti-inflammatory Role of Microsomal Prostaglandin E Synthase-1 in a Model of Neuroinflammation. J Biol. Chem.286: 2331-2342. Chaudhry et al . (2008) Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease. Alzhaimers Dement. 4:6-13. Jakobsson et al. (1999) Identification of human prostaglandin E synthase: a microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. PNAS 96:7220-7225.