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mPGES-1 glutathione dependent prostaglandin E synthase

444 €

AS03 031 | clonality: polyclonal  | host: rabbit | reactivity: human, mouse, sheep, rat

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Item No:
AS03 031

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product information
Background

mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.

Immunogen

Recombinant human purified 6-His mPGES-1 O14684

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB), Immunohistochemistry (IHC)
Related products

AS03 031S | mPGES-1 glutathione dependent prostaglandin E synthase protein | positive control

Secondary antibodies

Additional information

in our hands this antibody works with fresh frozen sections not in paraffin embeded material in IHC

mPGES-1 antibody was characterized using spleen tissue from LPS-induced mPGES-1 knock-out and wild type mice. In spleen tissue from wt mice we observed strong positive staining in macrophage-like cells, while there was no staining for mPGES-1 in ko mice.

application information
Recommended dilution

1:10 000 on human and rat samples (WB) 1:6 000 on  mice samples and in sheep seminal vesicles (IHC)

Expected | apparent MW

17 kDa

Confirmed reactivity human, mouse, rat, sheep
Predicted reactivity

dog, hamster, horse

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

antibody is only recognizing mPGES1 not mPGES2 or cPGES

This product can be sold containing proclin if requested.

Selected references Kern et al. (2017). CD200 selectively upregulates prostaglandin E2 and D2 synthesis in LPS-treated bone marrow-derived macrophages. Prostaglandins Other Lipid Mediat. 2017 Jun 3. pii: S1098-8823(17)30010-2. doi: 10.1016/j.prostaglandins.2017.06.002.
Bhatia et al. (2017). Alleviation of Microglial Activation Induced by p38 MAPK/MK2/PGE2 Axis by Capsaicin: Potential Involvement of other than TRPV1 Mechanism/s. Sci Rep. 2017 Dec;7(1):116. doi: 10.1038/s41598-017-00225-5.
Tuure et al. (2014). Aurothiomalate inhibits the expression of mPGES-1 in primary human chondrocytes. Scand J Rheumatol. 2014 Oct 14:1-6.
Olajide et al. (2014). Picralima nitida seeds suppress PGE2 production by interfering with multiple signalling pathways in IL-1β-stimulated SK-N-SH neuronal cells. J Ethnopharmacol. 2014 Jan 31. pii: S0378-8741(14)00074-9. doi: 10.1016/j.jep.2014.01.027.


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