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mPGES-1 glutathione dependent prostaglandin E synthase protein | positive control

177 €

AS03 031S  |  recombinant protein | positive control

PRODUCT INFORMATION IN PDF

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AS03 031S

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product information
Background

mPGES-1 is an inducible glutathione dependent prostaglandin E synthase that converts cyclooxygenase derived PGH2 into PGE2.

Host
Clonality
Clone
Purity
Format Liquid
Quantity 50 ĩl (20.5 ĩg)
Reconstitution
Storage

store protein standard at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB), immunolocalization (blocking protein for antibody)
Related products

AS03 031 | mPGES-1 glutathione dependent prostaglandin E synthase antibodies

Additional information

Protein is provided in 10 mM NaPI, pH 8, 150 mM KCl, 0.1 mM EDTA, 1mM GSH, 10 % glycerol, 0,1 % reduced Triton X-100. It has been produced in E.coli.

If intended to be used in Western blot, loading buffer should be added and the solution should be heated up in 98°C for 5 minutes before loading on the gel.

application information
Recommended dilution

positive control: 5 μl load per well is optimal for most chemiluminescent detection systems.

preabsorption:  6-12 ug of protein/100ul diluted anti-mPGES-1 serum (1:5000)

Expected | apparent MW

16-17 kDa

Confirmed reactivity
Predicted reactivity
Not reactive in

no confirmed exceptions from predicted reactivity currently known

Additional information

Concentration:  0.41 µg/µl.

Western blot: recommended load per gel to allow Coomasie staining and western blot detection is 5 µl

Immunolocalization: 6-12 ug of protein/100 µl diluted anti-mPGES-1 serum (1:5000) was used for preabsorption of mPGES-1 antibodies followed by immunolocalization.

Selected references

to be added when available



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