Animal cell antibodies / Human proteins / Oxidative stress

product information

Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.

8-hydroxy-guanosine-BSA and – casein conjugates

store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

ELISA (ELISA), immunostaining on frozen tissue and paraffin-embedded ((IHC-Fr-P), affinity purification (AP)

AS10 708-25 | hydroxy guanosine monoclonal antibody, 25 µg

protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml

application information

needs to be determined for each application

n.a.

Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)

does not apply

no confirmed exceptions from predicted reactivity known in the moment

protocol for immunostaining using this antibody can be found here

Pilger & Rudiger (2006). 8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures. Int Arch Occup Environ Health. 1: 1-15.

Kowluru et al. (2006). Role of mitochondrial superoxide dismutase in the development of diabetic retinopathy.Invest Ophthalmol Vis Sci 4: 1594-9.

Kim et al. (2003). Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells. Carcinogenesis 2: 235-241.

Cui et al. (2000). Oxidative DNA damage precedes DNA fragmentation after experimental stroke in rat brain. Faseb J. 14(7): 955-67.