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8-Hydroxyguanosine | DNA/RNA oxidative damage

383 €

AS10 708-100  | clonality: monoclonal  |  host: mouse  |  reactivity: hydroxy guanosine

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AS10 708-100

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product information
Background

Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.

Immunogen

8-hydroxy-guanosine-BSA and – casein conjugates

Host Mouse
Clonality Monoclonal
Clone IgG2A (clone 15A3)
Purity Protein G purified
Format Liquid
Quantity 100 ĩg
Reconstitution
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
Related products

AS10 708-25 | hydroxy guanosine monoclonal antibody, 25 µg

Secondary antibodies

Additional information Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml.
application information
Recommended dilution The optimal working dilution should be determined by the investigator.
Expected | apparent MW
Confirmed reactivity Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Protocol for immunostaining using this antibody can be found here.

Selected references Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048.
Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.


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