SOD1 aa 03-20 | superoxide dismutase 1, soluble
AS09 534 | clonality: polyclonal | host: rabbit | reactivity: human
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1 : 10 000 with standard ECL (WB)
|Expected | apparent MW||
bovine, goat, guinea pig, horse, mouse, pig, rat, sheep, crab, yeast
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Peptide used to elicit this antibody is not conserved in SOD2,3 and 4.
antibody available in November 2009
10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 534) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from DAKO PO448) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.
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