SOD1 aa 24-39 | superoxide dismutase 1, soluble
AS09 535 | clonality: polyclonal | host: rabbit | reactivity: human
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|Recommended dilution||1 : 10 000 (WB)|
|Expected | apparent MW||
|Predicted reactivity||Chimpanzee, Schizosaccharomyces pombe|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Peptide used to elicit this antibody is not conserved in SOD2,3 and 4.
antibody available in October 2009
10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 535) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from DAKO PO448) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.
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