Animal cell antibodies / Human proteins / Oxidative stress

product information

SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic

KLH-conjugated synthetic peptide, amino acids 80-96 derived from human SOD1 sequence P00441

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS09 534 | SOD1 aa 03-20 | superoxide dismutase 1, soluble

AS09 535 | SOD1 aa 24-39 | superoxide dismutase 1, soluble

AS09 536 | SOD1 aa 43-57 | superoxide dismutase 1, soluble

AS09 537 | SOD1 aa 58-72 | superoxide dismutase 1, soluble

AS09 539 | SOD1 aa 100-115 | superoxide dismutase 1, soluble

AS09 540 | SOD1 aa 131-153 | superoxide dismutase 1, soluble

to be added when available

application information

1 : 75 000 with standard ECL (WB)

15.9 kDa

human

bovine, chimpanzee, dog, goat, guinea pig, mouse, pig, rabbit, rat, sheep, fission yeast

no confirmed exceptions from predicted reactivity known in the moment

Peptide used to elicit this antibody is not conserved in SOD2,3 and 4.

antibody available in October 2009

application example 

 10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 538) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from DAKO PO448) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.