Animal cell antibodies / Neurosteroids/Neurobiology

product information

Glycine (Gly) is a smallest of amino acids found in proteins. 

Synthetic glycine conjugated to protein carrier.

store lyophilized antibodies at  4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

immunocytochemistry (ICC), immunohistochemistry (IHC), Dot blot (Dot)

to be added when available

Compounds                          Cross-reactivity ratio (a)

Glycine-G-PC*                                       1
β−Alanine-G-PC                                  1/100
GABA-G-PC                                       1/500
Taurine-G-PC                                     1/1,000
L.Aspartate-G-PC                               1/20,000
L.Glutamate-G-PC                              1/> 20,000
Free Glycine                                        1/> 50,000
 

G- glutaraldehyde, (a) Glycine-G-PC concentration/unconjugated or conjugated amio acid concentration at half displacement

application information

1: 1000 - 1: 5 000 (ICC)

n.a.

glycine

glycine

no confirmed exceptions from predicted reactivity known in the moment

for example of immunocytochemistry protocol check here.

Rubio & Juiz (2004). Differential distribution of synaptic endings containing glutamate, glycine, and GABA in the rat dorsal cochlear nucleus. J. Comp. Neurol. 477: 253-272. Van Zundert et al. (2000). Effects of chronic ethanol treatment on gamma-aminobutyric acid(A) and glycine receptors in mouse glycinergic spinal neurons. J. Pharmacol. Exp. ther. 1:423-429.