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Gly | glycine

418 €

AS09 593  |  clonality: polyclonal  |  host: rabbit

PRODUCT INFORMATION IN PDF

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AS09 593

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product information
Background

Glycine (Gly) is a smallest of amino acids found in proteins. 

Immunogen

Synthetic glycine conjugated to protein carrier.

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity 100 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water and 50 ĩl of glycerol
Storage

store lyophilized antibodies at  4°C; after reconstitution stode at -20°C. This aliquote is stable for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications immunocytochemistry (ICC), immunohistochemistry (IHC), Dot blot (Dot)
Related products

Secondary antibodies

Additional information
Compounds Cross-reactivity ratio (a)
   
Glycine-G-PC* 1
β−Alanine-G-PC 1/100
GABA-G-PC 1/500
Taurine-G-PC 1/1 000
L.Aspartate-G-PC 1/20 000
L.Glutamate-G-PC 1/>20 000
Free Glycine 1/>50 000

G- glutaraldehyde, (a) Glycine-G-PC concentration/unconjugated or conjugated amio acid concentration at half displacement

application information
Recommended dilution

1: 1000 - 1: 5 000 (ICC)

Expected | apparent MW
Confirmed reactivity

glycine

Predicted reactivity

glycine

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

for example of immunocytochemistry protocol check here.
total IgG was purified by ammonium sulfate precipitation

Selected references

Rubio & Juiz (2004). Differential distribution of synaptic endings containing glutamate, glycine, and GABA in the rat dorsal cochlear nucleus. J. Comp. Neurol. 477: 253-272. Van Zundert et al. (2000). Effects of chronic ethanol treatment on gamma-aminobutyric acid(A) and glycine receptors in mouse glycinergic spinal neurons. J. Pharmacol. Exp. ther. 1:423-429.



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