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NGF | Nerve growth factor

350 €

AS13 2692 | clonality: polyclonal | host: rabbit | reactivity: mouse

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AS13 2692

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Background

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. Homodimer, associated by noncovalent forces. It belongs to the NGF-beta family.

Immunogen

Native mouse beta NGF purified from submaxillary salivary gland.

Host Rabbit
Clonality Polyclonal
Clone
Purity Protein G purified IgG
Format Lyophilized
Quantity 500 ĩl
Reconstitution For reconstitution add 500 ĩl of sterile water.
Storage

After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Tested applications Immunohistochemistry (IHC), 1-site ELISA, Western blot (WB), immunoblot.
Related products
Additional information
application information
Recommended dilution

A concentration of 1-3 µg/ml is recommended for IHC, western blot and immunoblot, and ELISA. Use neat for in vivo studies at 2-10 µg/ml (ED50). This antibody was tested on cultured sensory neurons supported by 100ng/ml of purified mouse beta NGF. Be advised that 2ug/ml will neutralize 100ng/ml of mouse NGF. The higher 10ug/ml is only recommended if the concentration of NGF being used is higher than 100ng/ml such as the 200 or 500ng/ml that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Optimal dilutions is determined by the end user.

Expected | apparent MW
Confirmed reactivity

rat, mouse, human

Predicted reactivity
Not reactive in

bovine

Additional information

to be added when available

Selected references

to be added when available











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