THP | Allopregnanolone

Allopregnanolone, (3alpha-hydroxy-5alpha-pregnan-20-ol), also known as 3alpha,5alpha-tetrahydroprogesterone or THP, is a neuroactive steroid secreted by the mammalian ovary and adrenal. It exerts its anesthetic, anxiolytic, and sedative/hypnotic effects through potentiation of GABA A receptors and its release is stimulated by trophic hormones in relation to stress and reproductive hormone secretion.

BSA conjugated-allopregnanolone

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

radioimmunoassay (RIA)

AS04 041S | Allopregnanolone steroid standard

100 µl will be enough to perform 60 double samples, generating standard curve and blank

Waters column Symmetry C18 3.5um 4.6 x 75mm with 3.9 x 20mm as guard column has been used