Goat anti-rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity to bovine,human,mouse IgG/serum

187 €

AS10 1146 | clonality: polyclonal | host:goat | reactivity:rabbit IgG (H&L)

product information

background

Goat anti-rabbit IgG (H&L), HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

immunogen

antibody format

goat

polyclonal,

affinity purified IgG, F(ab)'2 fragment,

lyophilized

quantity

0.5 mg

storage

Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

related products

other HRP conjugated secondary antibodies to rabbit IgG

additional information

This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, human or mouse IgG from human or mouse.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

application information
recommended dilution		1:500-1: 5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.
selected references