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product information
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| background |
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Goat anti-biotin is a secondary antibody conjugated to DyLight®488, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. DyLight®488 has Amax = 493 nm; Emax = 518 nm. |
| antibody format |
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| goat |
polyclonal, |
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affinity purified IgG, |
lyophilized |
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| quantity |
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| storage |
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Store freeze dried powder at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C. Rehydrate with 1.1 ml of deionized water. Prepare fresh working dilution daily. |
| related products |
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other secondary antibodies against biotin
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| recommended dilution |
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This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2000 for most applications. |
| additional information |
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Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
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