Rabbit anti-guinea pig IgG (H&L), ALP conjugated

165 €

AS10 1493 | clonality: polyclonal | host: rabbit | reactivity: guinea pig IgG (H&L)

product information

background

Rabbit anti-guinea pig IgG (H&L) is a secondary antibody conjugated to ALP, which binds to guinea pig IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase guinea pig IgG (H&L).

immunogen

Purified guinea pig IgG (H&L) NP_001166520

antibody format

rabbit

polyclonal,

affinity purified rabbit IgG,

liquid

quantity

0.5 mg

storage

Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

related products

other secondary antibodies against guinea pig IgG (H&L)

additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on guinea pig IgG, light chains on all guinea pig immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin guinea pig serum proteins.

application information
recommended dilution		This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2000 for Immunohistochemistry 1:50 000-1:5 000 for ELISA/Western blot.
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