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AP2 | Floral homeotic protein APETALA 2

265 €
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AS12 2609 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Oryza sativa

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AS12 2609

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product information
Background

AP2 (floral homeotic protein APETALA 2) is a transcription factor involved in the specification of floral organ identity, establishment of floral meristem identity and controlling seed mass. 

Immunogen

KLH-conjugated synthetic peptide derived from AP2 sequence of Arabidopsis thaliana, UniProt: P47927, TAIR: At4g36920

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AS11 1773 | WUSCHEL-related homeobox 4, rabbit antibody

AS11 1759 | WUS | WUSCHEL protein, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
Affinity purified antibodies are lyophilized from PBS pH 7.4 with 0.02 % sodium azide.
application information
Recommended dilution

1: 1000 - 1 : 6000 (WB)

Expected | apparent MW

47.8 kDa

Confirmed reactivity Arabidopsis thaliana, Brassica napus, Oryza sativa
Predicted reactivity
Arabis alpina, Lepidium appelianum
Not reactive in Nicotiana tabacum, Vitis vinifera
Additional information
This antibody was used in western blots on Lepidium appelianum and Lepidium campestre AP2 Protein expressed in E. coli cells. Applied dilution was 1: 6000.
Selected references

To be added when available, antibody released in December 2013


Application example

western blot using anti-AP2 antibodies
1 µg of total protein from Oryza sativa lines grown under drought stress conditions and over expressing protein DREB (AP2 domain, 285 aa, 31.35 kDa) (lines 1-5) and purified 100 ng of DREB (line 6), were separated on 12.5 % SDS-PAGE and blotted 4h to Nitrocellulose membrane. Blots were blocked with blocking agent 5% BSA or 1X TBS, 0.1% Tween-20 with 5% w/v non-fat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 6 000 for 1h at RT or overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 x 5 min in 1XTBST at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:8000 in for 1h at RT or max. 2h with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was preferred between 20 to 30 seconds or max. 1 min.

Courtesy of Dr. Sunita Yadav, Indian Institute of Technology Kharagpur, India

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com