APX | ascorbate peroxidase (algal)

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AS15 2992  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii


17 st
Item No:
AS15 2992

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product information

APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water.

Immunogen KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii APX sequence, UniProt: O49822
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS06 180 | anti-cAPX

Additional information
application information
Recommended dilution

1: 9000 with ECL (WB)

Expected | apparent MW

35 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Coccomyxa subellipsoidea C-169, Micromonas pusilla (strain CCMP1545) , Ostreococcus lucimarinus (strain CCE9901), Ulva fasciata, Volvox carteri

Not reactive in
Additional information no confirmed exceptions from predicted reactivity are currently known
Selected references to be added when available, antibody released in October 2015

application information

western blot using anti-algal AOX antibodies

25 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS / 50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2,500 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma) diluted to 1:25,000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed with ECL (Amersham) according to the manufacturer's instructions. Exposure time was typically 30 seconds.

Courtesy of Dr. Thomas Roach, University of Innsbruck, Austria

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