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ASY1 | Asynaptic phenotype protein 1

356 €

AS08 323  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Barchypodium distachyonTriticum aestivum

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Item No:
AS08 323

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Background

Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis. Synapsis is complete at pachytene following the formation of a tri-partite proteinaceous structure known as the synaptonemal complex (SC). In yeast, HOP1 is essential for formation of the SC, and localises along chromosome axes during prophase I. Homologues in Arabidopsis (AtASY1), Brassica (BoASY1) and rice (OsPAIR2) have been isolated through analysis of mutants that display decreased fertility due to severely reduced synapsis of homologous chromosomes. Analysis of these genes has indicated that they play a similar role to HOP1 in pairing and formation of the SC through localisation to axial/lateral elements of the SC.

Immunogen

Recombinant, full length, ASY1 protein produced in E.coli prepared from the sequence of Triticum aestivum ASY1 A7TVU8

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA), immunolocalization (IL)
Related products

AS08 338 | anti-ASY1 mouse antibody

Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB) , 1: 10 000 (ELISA), 1: 150 (IL)

Expected | apparent MW

66.3 kDa

Confirmed reactivity

Brachypodium distachyon, Triticum aestivum

Predicted reactivity Oryza sativa
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

Boden et al. (2007). Expression and functional analysis of TaASY1 during meiosis of bread wheat (Triticum aestivum).BMC Mol. Biol. 8:65



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