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product information
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| background |
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ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. |
| immunogen |
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purified native protein from spinach |
| antibody format |
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rabbit; |
polyclonal; |
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serum; |
lyophilized |
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| quantity |
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100 µl |
- for reconstitution add 100 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB) |
| related products |
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AS08 304 | anti-ATP synthase subunit alpha antibody AS03 030 | anti-ATP synthase subunit beta hen antibody AS08 312 | anti-ATP synthase subunit gamma antibody AS05 071 | anti-ATP synthase subunit c antibody |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 10 000 (WB) |
| expected | apparent MW |
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38-58 kDa |
| confirmed reactivity |
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subunits alpha, beta, gamma, epsilon of Ch. reinhardtii, S. oleracea, P.sativum, perennial grasses: Eremochloa ophiuriudes, Paspalum vaginatum
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| predicted reactivity |
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n. a. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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the prominent reaction is to a doublet around 55-58 kDa which is alpha and beta and weaker reaction to gamma subunit which is around 38 kDa |
| selected references |
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Liu et al. (2011). Identification of differentially expressed salt-responsive proteins in roots of two perennial grass species contrasting in salinity tolerance. J Plant Physiol. Nov 7 |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com
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